Quickstart Highlights

Tri-Heal is a research peptide blend combining TB-500 (25 mg), BPC-157 (10 mg), and KPV (10 mg) for advanced tissue repair and inflammation modulation^[1]. TB-500 is a synthetic fragment of thymosin β4, a 43-amino-acid peptide involved in cell migration, angiogenesis, and wound healing^[2]. BPC-157 is a synthetic 15-amino-acid body protection compound with pro-angiogenic and gastroprotective properties^[3]. KPV (Lys-Pro-Val) is a tripeptide derived from α-MSH noted for potent anti-inflammatory effects^[4]. This educational protocol presents a once-daily subcutaneous approach using a practical dilution for clear insulin-syringe measurements.

• Reconstitute: Add 3.0 mL bacteriostatic water → 15 mg/mL concentration (15,000 mcg/mL).
• Typical daily range: 500–2000 mcg once daily (gradual titration over 8 weeks).
• Easy measuring: At 15 mg/mL, 1 unit = 0.01 mL = 150 mcg on a U-100 insulin syringe.
• Storage: Lyophilized: freeze at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F); avoid freeze–thaw cycles^[5].

Protocol Overview

Concise summary of the once-daily regimen.

• Goal: Support tissue repair, regeneration, and inflammation modulation through synergistic peptide action^[2][3][4].
• Schedule: Daily subcutaneous injections for 8–12 weeks (extend to 16 weeks if desired).
• Dose Range: 500–4000 mcg daily with gradual titration.
• Reconstitution: 3.0 mL per 45 mg vial (15 mg/mL) for accurate unit measurements.
• Storage: Lyophilized frozen; reconstituted refrigerated; avoid repeated freeze–thaw^[5].

Dosing Protocol

Suggested daily titration approach.

• Start: 500 mcg daily; increase by 500 mcg every 2 weeks as tolerated.
• Target: 1500–2000 mcg daily by Weeks 5–8 (standard); up to 3000–4000 mcg for extended protocols.
• Frequency: Once per day (subcutaneous).
• Cycle Length: 8–12 weeks; optional extension to 16 weeks.
• Timing: Any consistent time; rotate injection sites systematically.

Storage Instructions

Proper storage preserves peptide quality and potency.

• Lyophilized: Store at −20 °C (−4 °F) or colder (ideally −80 °C / −112 °F for long-term) in dry, dark conditions^[5].
• Reconstituted: Refrigerate at 2–8 °C (35.6–46.4 °F); use within 4 weeks; prepare aliquots if needed and avoid freeze–thaw cycles^[5].
• Allow vials to reach room temperature before opening to reduce condensation uptake.

Important Notes

Practical considerations for consistency and safety.

• Use new sterile insulin syringes for each injection; dispose in a sharps container.
• Rotate injection sites (abdomen, thighs, upper arms) to reduce local irritation and prevent lipohypertrophy^[6].
• Inject slowly; wait a few seconds before withdrawing the needle.
• Document daily dose, site rotation, and any observations to maintain consistency.
• For doses under 10 units, use 30-unit or 50-unit insulin syringes for improved accuracy.

How This Works

Tri-Heal combines three peptides with complementary mechanisms targeting multiple aspects of tissue injury repair:

TB-500 (Thymosin β4): Acts as a natural repair factor, inhibiting inflammation and apoptosis while promoting angiogenesis, cell migration, and matrix remodeling^[2][7]. This 43-amino-acid peptide has been shown to accelerate dermal healing in both preclinical models and clinical applications^[2].

BPC-157 (Body Protection Compound): Enhances healing via VEGFR2/Akt-eNOS activation, increasing nitric oxide production and angiogenesis while stimulating fibroblast activity^[8]. This 15-amino-acid gastric peptide accelerates tendon, muscle, and gut repair^[3][9].

KPV (Lys-Pro-Val): Provides anti-inflammatory effects by reducing NF-κB activation and attenuating pro-inflammatory cytokine signaling in epithelial and immune cells^[4]. This α-MSH-derived tripeptide complements the regenerative actions of TB-500 and BPC-157.

The combination thus addresses wound repair, angiogenesis, and inflammation simultaneously through distinct but synergistic pathways.

Potential Benefits & Side Effects

Observations from preclinical and clinical literature on the individual components.

• TB-500: Promotes cell proliferation, angiogenesis, and accelerated tissue repair; inhibits apoptosis and inflammation in healing tissues^[2][7].
• BPC-157: Accelerates musculoskeletal healing including tendon and muscle repair; stimulates endothelial regeneration and fibroblast activity^[3][9].
• KPV: Reduces inflammatory cytokine release; demonstrates efficacy in models of epithelial inflammation^[4].
• General tolerability: Individual peptides are generally well tolerated in preclinical studies; occasional mild injection-site reactions (redness, transient discomfort) may occur with subcutaneous administration.

Note: Human clinical data on this specific combination is limited; observations are extrapolated from studies of individual components.

Lifestyle Factors

Complementary strategies for optimal recovery outcomes.

• Pair with adequate protein intake (1.6–2.2 g/kg body weight) to support tissue regeneration.
• Maintain appropriate activity levels; avoid overloading healing tissues while promoting controlled movement.
• Prioritize sleep (7–9 hours) to maximize endogenous repair processes.
• Manage stress through appropriate techniques, as chronic stress impairs healing.
• Stay well-hydrated and maintain balanced micronutrient intake (vitamin C, zinc, collagen precursors).

Injection Technique

General subcutaneous guidance from clinical best-practice resources^[6].

• Clean the vial stopper and skin with alcohol; allow to dry completely before proceeding.
• Pinch a skinfold; insert the needle at 45–90° into subcutaneous tissue^[6][10].
• Do not aspirate for subcutaneous injections; inject slowly and steadily^[6].
• Rotate sites systematically (abdomen, thighs, upper arms) to avoid lipohypertrophy and local irritation^[11].
• Dispose of used syringes in an approved sharps container; never recap or reuse needles.

Recommended Source

We recommend Pure Lab Peptides for high-purity Retatrutide (5 mg vials).

Note: Product availability and specifications subject to change. Verify current product details on supplier website.

Shop at Pure Lab Peptides

References