Peptide Blends

BPC157 + TB500 (10mg Blend Vial) Dosage Protocol

BPC157 + TB500 (10mg Blend Vial) Dosage Protocol

Quickstart Highlights

This blend combines two well-studied regenerative peptides: BPC-157 (Body Protection Compound-157), a pentadecapeptide derived from human gastric juice with cytoprotective and wound-healing properties[1][2], and TB-500 (Thymosin Beta-4 fragment), a 43-amino-acid peptide involved in tissue repair, cell migration, and angiogenesis[3][4]. This educational protocol presents a once-daily subcutaneous approach using a practical dilution for clear insulin-syringe measurements.

  • Reconstitute: Add 3.0 mL bacteriostatic water → 3.33 mg/mL total concentration (1.67 mg/mL of each peptide).
  • Typical daily range: 600–1000 mcg total blend once daily (provides 300–500 mcg of each peptide).
  • Easy measuring: At 3.33 mg/mL, 1 unit = 0.01 mL ≈ 33.3 mcg total blend on a U-100 insulin syringe.
  • Storage: Lyophilized: freeze at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F); avoid freeze–thaw cycles.
BPC-157TB-500_Product_display-1

 Dosing & Reconstitution Guide

Standard / Gradual Approach (3 mL = ~3.33 mg/mL)

Route: Subcutaneous injection, once daily[5][6]. This blend contains equal amounts of BPC-157 and TB-500 (5 mg each); every dose drawn provides a 1:1 ratio of both peptides.

Phase / Week Daily Dose (Total Blend) Each Peptide (mcg) Units (mL)
Weeks 1–2 (Initial) 600 mcg 300 mcg BPC + 300 mcg TB-500 18 units (0.18 mL)
Weeks 3–4 (Loading) 800 mcg 400 mcg BPC + 400 mcg TB-500 24 units (0.24 mL)
Weeks 5–8 (Maintenance) 600 mcg 300 mcg BPC + 300 mcg TB-500 18 units (0.18 mL)

Frequency: Inject once daily subcutaneously. This schedule uses 3.0 mL dilution to keep per-injection volumes ≥18 units for accurate measurement. Rotate injection sites systematically[7].

Reconstitution Steps

    1. Draw 3.0 mL bacteriostatic water with a sterile syringe.
    2. Inject slowly down the vial wall; avoid foaming or vigorous shaking.
    3. Gently swirl/roll until fully dissolved (do not shake).
    4. Label with date and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.

Advanced / Aggressive Approach (Acute Injury Support)

For acute tissue injuries, some protocols suggest a higher initial loading phase[8][9]. This approach uses higher daily doses during the first 4 weeks before tapering to maintenance. Use with caution; robust human dose-finding data remain limited.

Phase / Week Daily Dose (Total Blend) Each Peptide (mcg) Units (mL)
Weeks 1–2 (Aggressive Load) 1000 mcg 500 mcg BPC + 500 mcg TB-500 30 units (0.30 mL)
Weeks 3–4 (High Load) 800 mcg 400 mcg BPC + 400 mcg TB-500 24 units (0.24 mL)
Weeks 5–8 (Maintenance) 600 mcg 300 mcg BPC + 300 mcg TB-500 18 units (0.18 mL)

Note: A small human case series combining BPC-157 and TB-500 for joint injuries reported improved outcomes at higher combined doses (4 mg BPC + 6 mg TB-500 intra-articular) compared to lower doses[8]. However, systemic subcutaneous protocols typically use the ranges above. Treatment duration is generally limited to 8–12 weeks before cycling off to evaluate response[9].

Important: This guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.

Supplies Needed

Plan based on an 8–16 week daily protocol with phased dosing (averaging ~700 mcg/day over the cycle).

  • Peptide Vials (BPC-157 + TB-500, 10 mg blend each):
    • 8 weeks ≈ 4 vials
    • 12 weeks ≈ 6 vials
    • 16 weeks ≈ 8 vials
  • Insulin Syringes (U-100):
    • Per week: 7 syringes (1/day)
    • 8 weeks: 56 syringes
    • 12 weeks: 84 syringes
    • 16 weeks: 112 syringes
  • Bacteriostatic Water (10 mL bottles): Use 3.0 mL per vial for reconstitution.
    • 8 weeks (4 vials): 12 mL2 × 10 mL bottles
    • 12 weeks (6 vials): 18 mL2 × 10 mL bottles
    • 16 weeks (8 vials): 24 mL3 × 10 mL bottles
  • Alcohol Swabs: One for the vial stopper + one for the injection site each day.
    • Per week: 14 swabs (2/day)
    • 8 weeks: 112 swabs → recommend 2 × 100-count boxes
    • 12 weeks: 168 swabs → recommend 2 × 100-count boxes
    • 16 weeks: 224 swabs → recommend 3 × 100-count boxes

Protocol Overview

Concise summary of the once-daily regimen.

  • Goal: Support tissue repair, wound healing, and recovery from musculoskeletal injuries[1][3].
  • Schedule: Daily subcutaneous injections for 8–12 weeks (extend to 16 weeks if needed).
  • Dose Range: 600–1000 mcg total blend daily (300–500 mcg of each peptide).
  • Reconstitution: 3.0 mL per 10 mg vial (~3.33 mg/mL) for accurate unit measurements.
  • Storage: Lyophilized frozen; reconstituted refrigerated; avoid repeated freeze–thaw.

Dosing Protocol

Suggested daily phased approach.

  • Start: 600 mcg total daily (300 mcg each peptide) for Weeks 1–2.
  • Loading: Increase to 800 mcg daily for Weeks 3–4.
  • Maintenance: Return to 600 mcg daily for Weeks 5–8+.
  • Frequency: Once per day (subcutaneous).
  • Cycle Length: 8–12 weeks; optional extension to 16 weeks.

Storage Instructions

Proper storage preserves peptide quality and stability.

  • Lyophilized: Store at −20 °C (−4 °F) in dry, dark conditions; minimize moisture exposure.
  • Reconstituted: Refrigerate at 2–8 °C (35.6–46.4 °F); use within 4–6 weeks; avoid freeze–thaw.
  • Allow vials to reach room temperature before opening to reduce condensation uptake.

Important Notes

Practical considerations for consistency and safety.

  • Both peptides are not approved for routine human use (sold for research purposes) and are banned in competitive sports[10].
  • Use new sterile insulin syringes for each injection; dispose in a sharps container.
  • Rotate injection sites (abdomen, thighs, upper arms) to reduce local irritation[7].
  • Inject slowly; wait a few seconds before withdrawing the needle.
  • Document daily dose and site rotation to maintain consistency.

How This Works

BPC-157 is a stable pentadecapeptide that promotes angiogenesis, modulates nitric oxide pathways, and demonstrates broad cytoprotective effects across gastrointestinal, musculoskeletal, and neurological tissues in preclinical models[1][2]. It has shown activity at very low doses (nanogram to microgram per kilogram) without demonstrable toxicity in animal studies[11].

TB-500 (Thymosin Beta-4) is an actin-sequestering peptide that promotes cell migration, wound healing, and anti-inflammatory responses[3][4]. It is well-tolerated in animal and early clinical studies, even at multi-milligram doses[12].

Combining these peptides may provide complementary mechanisms for tissue repair: BPC-157 for its trophic and anti-inflammatory effects, and TB-500 for enhanced cell migration and angiogenesis[8].

Potential Benefits & Side Effects

Observations from preclinical and limited clinical literature.

  • May support accelerated healing of tendons, ligaments, muscles, and soft tissue injuries[1][5].
  • Preclinical evidence suggests gastroprotective and anti-inflammatory properties for BPC-157[2].
  • TB-500 promotes wound healing and may reduce scarring through enhanced cell migration[3].
  • Both peptides are generally well tolerated; occasional mild injection-site reactions (redness, itching) may occur with subcutaneous administration.
  • No significant toxicity has been documented up to high doses in animal studies[11][12].

Lifestyle Factors

Complementary strategies for optimal recovery outcomes.

  • Prioritize adequate protein intake (1.6–2.2 g/kg body weight) to support tissue repair.
  • Follow appropriate rehabilitation protocols for any injuries being addressed.
  • Ensure adequate sleep (7–9 hours) to optimize recovery and tissue regeneration.
  • Manage inflammation through balanced nutrition and stress reduction techniques.

Injection Technique

General subcutaneous guidance from clinical best-practice resources[6][7].

  • Clean the vial stopper and skin with alcohol; allow to dry completely.
  • Pinch a skinfold; insert the needle at 45–90° into subcutaneous tissue[13].
  • Do not aspirate for subcutaneous injections; inject slowly and steadily[13].
  • Rotate sites systematically (abdomen at least 2 inches from navel, thighs, upper arms, flank) to avoid lipohypertrophy[7].
  • Apply gentle pressure post-injection; do not rub the site.

Recommended Source

We recommend Pure Lab Peptides for high-purity BPC-157 + TB-500 (20 mg Blend).

Why Pure Lab Peptides?

  • High-purity (≥99% HPLC), third-party-tested lots with batch COAs.
  • Consistent, ISO-aligned handling and documentation.
  • Reliable fulfillment to maintain cold-chain integrity.

Shop at Pure Lab Peptides

Important Note

This content is intended for therapeutic educational purposes only and does not constitute medical advice, diagnosis, or treatment.

References

PubMed
— Preclinical safety evaluation of body protective compound-157, a potential drug for treating various wounds
Current Pharmaceutical Design (PubMed)
— Stable gastric pentadecapeptide BPC-157: novel therapy in gastrointestinal tract
PMC
— Utilizing developmentally essential secreted peptides such as Thymosin Beta-4 for regenerative therapies
Annals of the New York Academy of Sciences (PubMed)
— Thymosin beta 4 and wound healing: new ideas
PMC
— Emerging use of BPC-157 in orthopaedic sports medicine: a systematic review
Johns Hopkins Arthritis Center
— How to give a subcutaneous injection
PMC
— Subcutaneous drug delivery review: pharmacologic considerations
A4M
— Thymosin Beta-4 professional monograph
PubMed
— Detection of thymosin beta-4 and related peptides in sport drug testing
Journal of Physiology and Pharmacology (PubMed)
— BPC-157 activity at very low doses without toxicity in animal studies
Expert Opinion on Biological Therapy (PubMed)
— Thymosin beta-4 and its role in wound healing
CDC
— Vaccine administration: subcutaneous route (angle/site; no aspiration)
NCBI Bookshelf
— Best practices for injection (asepsis, preparation, and administration)
Pure Lab Peptides
— BPC-157 + TB-500 (10 mg blend) product page (quality and batch documentation)