Quickstart Highlights

GHK-Cu dosage protocols utilize this naturally occurring copper-binding tripeptide (glycyl-L-histidyl-L-lysine) to support skin rejuvenation, wound healing, and tissue repair^[1][2]. GHK-Cu stimulates collagen and elastin production, promotes angiogenesis, and delivers powerful antioxidant and anti-inflammatory benefits^[3][4]. Human studies demonstrate improved skin thickness, reduced wrinkles, and enhanced dermal density with GHK-Cu application^[2]. This educational protocol presents a once-daily subcutaneous approach using a practical dilution for clear insulin-syringe measurements.

• Reconstitute: Add 3.0 mL bacteriostatic water → ~33.3 mg/mL concentration.
• Typical daily range: 1–2 mg once daily (gradual titration over 12 weeks).
• Easy measuring: At 33.3 mg/mL, 1 unit = 0.01 mL ≈ 333 mcg on a U-100 insulin syringe.
• Storage: Lyophilized: freeze at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F); use within 30 days.

Protocol Overview

Concise summary of the once-daily regimen.

• Goal: Support skin rejuvenation, collagen synthesis, wound healing, and anti-aging tissue repair^[2][3].
• Schedule: Daily subcutaneous injections, 5 days on / 2 days off, for 8–12 weeks.
• Dose Range: 1–2 mg daily with gradual titration.
• Reconstitution: 3.0 mL per 100 mg vial (~33.3 mg/mL) for accurate unit measurements.
• Storage: Lyophilized frozen at −20 °C; reconstituted refrigerated; use within 30 days.

Dosing Protocol

Suggested daily titration approach.

• Start: 1.0 mg daily for Weeks 1–4; increase to 1.5 mg for Weeks 5–8.
• Target: 2.0 mg daily by Weeks 9–12.
• Frequency: Once per day (subcutaneous), 5 days per week.
• Cycle Length: 8–12 weeks; take 2–4 weeks off before repeating^[5].
• Timing: Any consistent time; rotate injection sites.

Storage Instructions

Proper storage preserves peptide quality.

• Lyophilized: Store at −20 °C (−4 °F) in dry, dark conditions; stable for 12+ months^[6].
• Reconstituted: Refrigerate at 2–8 °C (35.6–46.4 °F); use within 30 days; avoid freeze–thaw^[7].
• Allow vials to reach room temperature before opening to reduce condensation uptake.

Important Notes

Practical considerations for consistency and safety.

• Use new sterile insulin syringes (30- or 50-unit recommended for small volumes); dispose in a sharps container.
• Rotate injection sites (abdomen, thighs, upper arms) to reduce local irritation^[8].
• Inject slowly; wait a few seconds before withdrawing the needle.
• Document daily dose and site rotation to maintain consistency.
• Reconstituted solution should appear clear with a blue tint (from copper); discard if cloudy or discolored.

How This Works

GHK-Cu functions as a “signal peptide” that triggers tissue repair processes by chelating copper(II) ions and delivering this essential trace metal to cells in a bioavailable form^[1][9]. Copper is a critical cofactor for enzymes involved in collagen synthesis (lysyl oxidase) and antioxidant defense (superoxide dismutase). Genomic studies show GHK-Cu modulates over 30% of human genes toward a regenerative, anti-aging phenotype^[2][10]. The peptide stimulates production of VEGF and bFGF in fibroblasts, promoting angiogenesis and blood flow to injured tissue^[3]. GHK-Cu also recruits immune cells to injury sites, increases collagen/elastin/glycosaminoglycan synthesis, and regulates matrix metalloproteinases for balanced tissue remodeling^[2][4].

Potential Benefits & Side Effects

Observations from preclinical and clinical literature.

• Skin Rejuvenation: Clinical studies show increased skin thickness, improved dermal collagen density, and reduced wrinkle depth with GHK-Cu application^[2].
• Wound Healing: Promotes angiogenesis, granulation tissue formation, and accelerated wound closure in preclinical models^[3][4].
• Anti-Inflammatory: Suppresses pro-inflammatory cytokines (TNF-α, IL-6) and provides antioxidant protection^[1][11].
• Hair Growth: Observed to enlarge hair follicle size and increase growth rate in studies^[2].
• Safety Profile: Excellent tolerability with no serious adverse effects noted; extremely wide safety margin in toxicology studies^[9][10].
• Occasional mild injection-site reactions (transient redness or stinging) may occur with subcutaneous administration.

Lifestyle Factors

Complementary strategies for best outcomes.

• Ensure adequate protein and vitamin C intake to support collagen synthesis.
• Maintain proper hydration—tissue repair processes require sufficient water.
• Consider complementary skincare routines (sunscreen, moisturizers) to protect treated skin.
• Prioritize sleep and stress management to support recovery and tissue regeneration.

Injection Technique

General subcutaneous guidance from clinical best-practice resources^[8].

• Clean the vial stopper and skin with alcohol; allow to dry.
• Pinch a skinfold; insert the needle at 45–90° into subcutaneous tissue^[8].
• Do not aspirate for subcutaneous injections; inject slowly and steadily.
• Wait 5–10 seconds before withdrawing; dispose of syringe in sharps container.
• Rotate sites systematically (abdomen, thighs, upper arms) to avoid lipohypertrophy.

Recommended Source

We recommend Pure Lab Peptides for high-purity Retatrutide (5 mg vials).

Note: Product availability and specifications subject to change. Verify current product details on supplier website.

Shop at Pure Lab Peptides

References