BPC-157 (5mg Vial) Dosage Protocol
Contents
Quickstart Highlights
BPC-157 (Body Protection Compound-157) is a synthetic 15-amino-acid peptide derived from a gastric protein sequence studied for tissue-healing and cytoprotective properties[1][2]. Preclinical models demonstrate accelerated wound repair and anti-inflammatory activity[3], though human clinical data remain limited to early-phase safety trials and small case reports[4][5]. This educational protocol presents a once-daily subcutaneous approach using a practical dilution for clear insulin-syringe measurements.
- Reconstitute: Add 3.0 mL bacteriostatic water → 1.67 mg/mL concentration.
- Typical daily range: 200–600 mcg once daily (gradual titration).
- Easy measuring: At 1.67 mg/mL, 1 unit = 0.01 mL ≈ 16.7 mcg on a U-100 insulin syringe.
- Storage: Lyophilized: freeze at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F); avoid freeze–thaw cycles.

Dosing & Reconstitution Guide
Standard / Gradual Approach (3 mL = ~1.67 mg/mL)
Route: Subcutaneous | Frequency: Once daily
| Week | Daily Dose (mcg) | Units (per injection) (mL) |
|---|---|---|
| Weeks 1–2 | 200 mcg (0.2 mg) | 12 units (0.12 mL) |
| Weeks 3–4 | 400 mcg (0.4 mg) | 24 units (0.24 mL) |
| Weeks 5–8+ | 600 mcg (0.6 mg) | 36 units (0.36 mL) |
Frequency: Inject once daily subcutaneously. This schedule uses the largest practical dilution (3.0 mL) to keep per-injection units well above 10 for better accuracy. Dosing is extrapolated from preclinical models[1][6]; human clinical validation remains limited.
Reconstitution Steps
- Draw 3.0 mL bacteriostatic water with a sterile syringe.
- Inject slowly down the vial wall; avoid foaming.
- Gently swirl/roll until dissolved (do not shake).
- Label and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.
Important: This guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.
Supplies Needed
Plan based on an 8–16 week daily protocol with gradual titration.
- Peptide Vials (BPC-157, 5 mg each):
- 8 weeks ≈ 6 vials (25.2 mg used)
- 12 weeks ≈ 9 vials (42 mg used)
- 16 weeks ≈ 12 vials (58.8 mg used)
- Insulin Syringes (U-100):
- Per week: 7 syringes (1/day)
- 8 weeks: 56 syringes
- 12 weeks: 84 syringes
- 16 weeks: 112 syringes
- Bacteriostatic Water (10 mL bottles): Use ~3.0 mL per vial for reconstitution.
- 8 weeks (6 vials): 18 mL → 2 × 10 mL bottles
- 12 weeks (9 vials): 27 mL → 3 × 10 mL bottles
- 16 weeks (12 vials): 36 mL → 4 × 10 mL bottles
- Alcohol Swabs: One for the vial stopper + one for the injection site each day.
- Per week: 14 swabs (2/day)
- 8 weeks: 112 swabs → recommend 2 × 100-count boxes
- 12 weeks: 168 swabs → recommend 2 × 100-count boxes
- 16 weeks: 224 swabs → recommend 3 × 100-count boxes
Protocol Overview
Concise summary of the once-daily regimen.
- Goal: Support tissue-healing and recovery processes based on preclinical evidence[1][3].
- Schedule: Daily subcutaneous injections for 8–12 weeks (extend to 16 weeks if desired).
- Dose Range: 200–600 mcg daily with gradual titration.
- Reconstitution: 3.0 mL per 5 mg vial (~1.67 mg/mL) for accurate unit measurements.
- Storage: Lyophilized frozen; reconstituted refrigerated; avoid repeated freeze–thaw.
Dosing Protocol
Suggested daily titration approach.
- Start: 200 mcg daily; increase by ~200 mcg every 2 weeks as tolerated.
- Target: 400–600 mcg daily by Weeks 5–8+.
- Frequency: Once per day (subcutaneous).
- Cycle Length: 8–12 weeks; optional extension to 16 weeks.
- Timing: Any consistent time; rotate injection sites.
Storage Instructions
Proper storage preserves peptide quality.
- Lyophilized: Store at −20 °C (−4 °F) in dry, dark conditions; minimize moisture exposure.
- Reconstituted: Refrigerate at 2–8 °C (35.6–46.4 °F); prepare aliquots if needed and avoid freeze–thaw.
- Allow vials to reach room temperature before opening to reduce condensation uptake.
Important Notes
Practical considerations for consistency and safety.
- Use new sterile insulin syringes for each injection; dispose in a sharps container[7].
- Rotate injection sites (abdomen, thighs, upper arms) to reduce local irritation[8].
- Inject slowly; wait a few seconds before withdrawing the needle.
- Document daily dose and site rotation to maintain consistency.
- BPC-157 human data are preliminary; clinical decisions should involve qualified healthcare providers.
How This Works
BPC-157 is a synthetic peptide corresponding to a partial sequence of human gastric juice protein[2]. Preclinical studies suggest it modulates nitric oxide pathways and growth-factor expression to promote angiogenesis and collagen deposition in damaged tissues[1][3]. Animal models report accelerated healing of gut, tendon, ligament, and muscle injuries[6]. A Phase I oral safety trial has been completed[9], and a small human case series reported improvements following intra-articular administration[5]; however, large-scale controlled human efficacy data are not yet available.
Potential Benefits & Side Effects
Observations from preclinical and early clinical literature.
- Supports tissue repair in gut, tendon, muscle, and skin injury models (animal data)[1][3].
- Demonstrates anti-inflammatory and cytoprotective properties in preclinical settings[6].
- Phase I safety studies report good tolerability with no serious adverse events at tested doses[9].
- Occasional mild injection-site reactions (redness, itch) may occur with subcutaneous administration.
- Long-term human safety and efficacy remain under investigation[4].
Lifestyle Factors
Complementary strategies for best outcomes.
- Support recovery with adequate protein intake and micronutrient-rich foods.
- Balance activity and rest to allow tissue adaptation without overuse.
- Prioritize sleep and stress management to enhance natural healing processes.
- Consult qualified healthcare providers for injury-specific rehabilitation guidance.
Injection Technique
General subcutaneous guidance from clinical best-practice resources[10][11].
- Clean the vial stopper and skin with alcohol; allow to dry.
- Pinch a skinfold; insert the needle at 45–90° into subcutaneous tissue[10].
- Do not aspirate for subcutaneous injections; inject slowly and steadily[10].
- Rotate sites systematically (abdomen, thighs, upper arms) to avoid lipohypertrophy[8].
- Discard used syringes immediately in a sharps container per WHO guidelines[7].
Recommended Source
We recommend Pure Lab Peptides for high-purity Retatrutide (5 mg vials).
Why Pure Lab Peptides?
- Third-party tested: Each batch includes Certificate of Analysis (COA) verifying purity and composition.
- Consistent quality: ISO-aligned manufacturing and handling standards ensure reliable product integrity.
- Cold-chain integrity: Temperature-controlled shipping and storage throughout fulfillment process.
- Research-grade purity: Suitable for educational and research applications requiring high-quality peptides.
Note: Product availability and specifications subject to change. Verify current product details on supplier website.
Important Note
This content is intended for therapeutic educational purposes only and does not constitute medical advice, diagnosis, or treatment.
References
— Klicek R et al. BPC-157 promotes colocutaneous fistula healing via NO-system modulation (rat model)
— Józwiak M et al. Multifunctionality and possible medical application of BPC-157: literature and patent review (2025)
— Sikiric P et al. BPC-157 stable gastric pentadecapeptide: novel therapy for wound healing and tissue repair
— Seiwerth S et al. BPC-157 and standard angiogenic growth factors: gastrointestinal tract healing and beyond
— Vasireddi N et al. Emerging use of BPC-157 in orthopaedic sports medicine: systematic review (2025)
— Chang CH et al. BPC-157 promotes tendon-to-bone healing in a rat rotator cuff model
— Guideline on safety-engineered syringes for IM, ID, and SC injections in health care settings (2016)
— How to give a subcutaneous injection (patient education resource)
— NCT02637284: PCO-02 Phase I safety and pharmacokinetics trial of oral BPC-157
— Vaccine administration: subcutaneous route (angle/site; no aspiration)
— Pharmacologic considerations of the subcutaneous route
— Sikiric P et al. Pentadecapeptide BPC-157: from GI tract to whole body healing
— BPC-157 (5 mg) product page (quality and batch documentation)

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