Quickstart Highlights
TB-500 & BPC-157 dosage protocol combines two research peptides often used together for synergistic tissue repair and recovery.
- TB-500: 2–2.5 mg twice weekly (4–5 mg/week) for Weeks 1–4, then once weekly
- BPC-157: 250–500 mcg/day (can be split into 1–2 daily doses)
- 6–8 week cycle (up to 12 for chronic injury), then 2–4 weeks off
- Reconstitute each 10 mg vial with 2 mL bacteriostatic water (5 mg/mL)
- Store the reconstituted solution at 2–8 °C (refrigerator), protected from light.
Dosing & Reconstitution Guide
TB-500 (10 mg Vial): Standard Approach
Reconstitute with 2 mL of bacteriostatic water to achieve a concentration of 5 mg/mL. Each full vial = 10 mg total.
| Week | Weekly Total | Per Injection | Units (mL) | Frequency |
|---|---|---|---|---|
| Weeks 1–4 (loading) | 4–5 mg/week | 2–2.5 mg | 40–50 units (0.4–0.5 mL) | 2× per week |
| Weeks 5–8 (maintenance; to 12 for chronic injury) | 2–2.5 mg/week | 2–2.5 mg | 40–50 units (0.4–0.5 mL) | 1× per week |
Loading splits 4–5 mg per week into two subcutaneous injections 3–4 days apart; maintenance drops to one injection per week. At 5 mg/mL a 2.5 mg dose is 0.5 mL (50 units) on a U-100 syringe. One 10 mg vial covers two weeks of loading or four weeks of maintenance.
- Draw 2.0 mL of bacteriostatic water into a sterile syringe.
- Inject slowly along the vial wall to minimize foam.
- Gently swirl the vial—avoid vigorous shaking.
- Store the reconstituted solution at 2–8 °C (refrigerator), protected from light, and use within 28 days.
BPC-157 (10 mg Vial): Standard Approach
Reconstitute with 2 mL of bacteriostatic water, resulting in 5 mg/mL. Each full vial = 10 mg total.
| Week | Daily Total (mcg) | Per Injection | Units (mL) | Frequency |
|---|---|---|---|---|
| Weeks 1–2 | 500 mcg/day | 250 mcg | 5 units (0.05 mL) | 2× per day |
| Weeks 3–8 | 250–500 mcg/day | 250–500 mcg | 5–10 units (0.05–0.10 mL) | 1× per day |
Split dosing in the first two weeks keeps each injection small; a single daily dose from Week 3 is the usual pattern. At 5 mg/mL, 500 mcg is 0.1 mL (10 units); use a 30- or 50-unit syringe for readability at or below 10 units. One 10 mg vial covers 20–40 days.
- Draw 2.0 mL of bacteriostatic water into a sterile syringe.
- Inject slowly along the vial wall to minimize foam.
- Gently swirl the vial—avoid vigorous shaking.
- Store the reconstituted solution at 2–8 °C (refrigerator), protected from light, and use within 28 days.
Combining TB-500 & BPC-157 in a Stack
You can run both peptides simultaneously:
- TB-500: 2–2.5 mg twice weekly (4–5 mg/week) for Weeks 1–4, then 2–2.5 mg once weekly for maintenance
- BPC-157: 250–500 mcg/day, as one dose or split into two
- Each peptide reconstituted in its own 10 mg vial at 2 mL (5 mg/mL); do not mix in the same syringe
Users often report synergistic recovery effects with both peptides. Administering them on the same day is fine; just use separate syringes and injection sites if desired.
Important: This guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.
Supplies Needed
Plan based on an 8-week cycle (4 weeks TB-500 loading, 4 weeks maintenance; BPC-157 daily throughout).
- Peptide Vials:
• TB-500 (10 mg vial): 8 weeks uses 24–30 mg → 3 vials (reconstitute one at a time; use within 28 days)
• BPC-157 (10 mg vial): 8 weeks uses 21–28 mg → 3 vials - Insulin Syringes (U-100; 30- or 50-unit for BPC-157): TB-500 12 injections + BPC-157 70 injections → 82 syringes over 8 weeks
- Bacteriostatic Water (30 mL): 2 mL per vial, 12 mL total → 1 bottle (discard 28 days after opening; a second bottle for weeks 5–8)
- Alcohol Swabs: 2 per injection → 2 × 100-count boxes
Protocol Overview
A concise weekly (TB-500) and daily (BPC-157) injection strategy.
- Goal: Tissue repair and accelerated recovery
- TB-500: 4–5 mg per week (2–2.5 mg twice weekly) for Weeks 1–4, then 2–2.5 mg once weekly
- BPC-157: 250–500 mcg per day, split into 1–2 doses
- Cycle Length: 6–8 weeks (up to 12 for chronic injury), then 2–4 weeks off
- Reconstitution: 2 mL per 10 mg vial (5 mg/mL) for both peptides
Dosing Protocol
Recommended approach for combining both peptides effectively.
- Injection Method: Subcutaneous, rotating sites
- TB-500 Schedule: 2 injections per week (e.g., Monday & Thursday) for Weeks 1–4, then 1 per week
- BPC-157 Schedule: Daily or split daily for Weeks 1–4, optional taper afterward
- Timing: Separate syringes; no need for precise timing alignment
- Duration: 6–8 weeks, re-assess; up to 12 weeks for chronic injury, then 2–4 weeks off
Storage Instructions
Proper handling preserves peptide quality.
- Lyophilized: Keep frozen until ready to mix
- Reconstituted: Store at 2–8°C; use within 30 days
- Protect from light; keep vials upright if possible
- Avoid repeated freeze-thaw cycles
Important Notes
Tips for safe, consistent usage.
- Use new, sterile insulin syringes every time; rotate injection sites.
- Maintain consistent protocol; peptides often show best results with regular use.
- Monitor for adverse reactions; consult a professional if needed.
- Do not mix TB-500 and BPC-157 in the same syringe.
How This Works
Both peptides are known for supporting tissue repair in various research settings.
- TB-500 (Thymosin Beta-4 Fragment): May aid in cell migration and angiogenesis.
- BPC-157: Investigated for potential benefits in gut healing and collagen synthesis.
- They work via distinct biological mechanisms but often stack well for synergistic effects.
Potential Benefits & Side Effects
Research suggests various therapeutic benefits, with some caution advised.
- May accelerate soft tissue repair and reduce inflammation
- Possible support for tendon, ligament, and muscle recovery
- Mild side effects reported: injection-site irritation, headache, dizziness
- Long-term safety remains under study
Lifestyle Factors
Simple habits can complement peptide usage.
- Adopt a balanced, protein-rich diet
- Engage in regular exercise; allow sufficient recovery
- Focus on stress management and adequate sleep
Injection Technique
Simple guidelines for safe daily injections.
- Clean vial rubber stopper & injection site with alcohol swabs
- Insert needle at a 45–90° angle into subcutaneous tissue
- Inject slowly & rotate sites (abdomen, thigh, etc.)
Recommended Source
We recommend Amino Labs for high-purity BPC-157 + TB-500 (20 mg Blend).
Why Amino Labs?
- High-purity (≥99% HPLC), third-party-tested lots with batch COAs.
- Consistent, ISO-aligned handling and documentation.
- Reliable fulfillment to maintain cold-chain integrity.
Important Note
This content is intended for therapeutic educational purposes only and does not constitute medical advice, diagnosis, or treatment.